---
title: "Find structural analogs"
description: "Fold a sequence with ESMFold, then search PDB and AlphaFold DB with Foldseek and BLAST for structural and sequence homologs."
tags:
  - recipes
  - structure
tools:
  - esmfold
  - blast
generated: true
source: quickActionConfig:foldseek-blast
---

> **Generated recipe**

> Seeded from a curated quick-action prompt. The prompt below runs the real
> routing — copy it, change the target, and run.

> **Recommended pairing**

> These tools are commonly run together — they cover complementary ground on the same input.

**Best fit:** search by structure — a structure goal.

**Try this prompt** — [open in a guest chat](/chat/new?guest=true&prompt=Fold%20the%20orphan%20GPCR%20GPR75%20(UniProt%3A%20Q9ULV1)%20with%20ESMFold%2C%20then%20search%20for%20structural%20analogs%20using%20Foldseek%20against%20PDB%20and%20AlphaFold%20DB%20-%20which%20solved%20GPCR%20structures%20share%20the%20closest%20TM%20helix%20arrangement%20despite%20low%20sequence%20identity%20(%3C30%25)%2C%20and%20could%20their%20ligand%20binding%20sites%20inform%20GPR75%20deorphanization%20screens%3F).

> Fold the orphan GPCR GPR75 (UniProt: Q9ULV1) with ESMFold, then search for structural analogs using Foldseek against PDB and AlphaFold DB - which solved GPCR structures share the closest TM helix arrangement despite low sequence identity (<30%), and could their ligand binding sites inform GPR75 deorphanization screens?

_A real `foldseek-blast` quick-action prompt — open it in a guest chat, then swap in your own sequence._

## The prompt

```text
Fold the orphan GPCR GPR75 (UniProt: Q9ULV1) with ESMFold, then search for structural analogs using Foldseek against PDB and AlphaFold DB - which solved GPCR structures share the closest TM helix arrangement despite low sequence identity (<30%), and could their ligand binding sites inform GPR75 deorphanization screens?
```

## What each tool contributes

- [esmfold](/docs/esmfold) — folds the query sequence, so Foldseek has a structure to search with
- **foldseek** — 3D structure similarity search across PDB and AlphaFold DB using structural alphabet encoding
- [blast](/docs/blast) — Sequence-based homology search for evolutionarily related proteins

## Why these tools pair

Foldseek finds structural homologs via 3Di alphabet encoding while BLAST identifies sequence homologs — structural search catches remote homologs with &lt;20% sequence identity that BLAST misses entirely, critical for finding druggable analogs of novel targets and identifying off-target selectivity risks

## Data flow

Every tool operates on the same input: `pdb_structure`.

## See also

- [Sequence Analysis](/docs/workflows/sequence-analysis) — the guided version: BLAST, ESMFold, and UniProt step by step.
- [Which tool?](/docs/which-tool) — match a single goal to a tool.
- [Workflows](/docs/workflows) — every workflow and recipe.
- [How the orchestrator picks tools](/docs/how-orchestration-works) — the same chain from a plain-language goal.