BioPhi outputs and interpretation
Humanness scores, classification, humanized sequences, and mutations from BioPhi.
BioPhi returns an OASis humanness score and percentile, a human/humanized/chimeric/murine classification, and a per-CDR and per-framework breakdown. In a humanization run it also returns the rewritten VH/VL sequences and the list of mutations it made. Read the classification and the per-region breakdown together: a merely "humanized" class with a low-humanness framework region points you straight at where more work is needed.
Humanness fields
| Field | Type | What it tells you |
|---|---|---|
humanness_score | float | Overall OASis humanness (0–1). |
oasis_percentile | float | OASis percentile rank (0–100). |
humanness_class | human | humanized | chimeric | murine | Classification from the OASis percentile. |
vh_humanness | float | Heavy-chain humanness. |
vl_humanness | float | null | Light-chain humanness. |
cdr_humanness | map region→float | Per-CDR humanness (e.g. CDR-H1). |
framework_humanness | float | Combined framework-region humanness. |
immunogenicity_risk | low | moderate | high | Predicted immunogenicity risk. |
Humanization fields
Present when the job ran in a humanization mode:
| Field | Type | What it tells you |
|---|---|---|
humanized_vh | string | null | Humanized heavy-chain sequence. |
humanized_vl | string | null | Humanized light-chain sequence. |
mutations_made | list | Mutations applied, each as { position, from_aa, to_aa }. |
immunogenicity_reduction_percent | float | null | Estimated immunogenicity reduction from humanization. |
Reading the result card
The BioPhi result renders on the canvas as a humanization card, surfacing the humanness score, class, and (in humanization mode) the mutations so you can see the change at a glance.
What to do next
- Feed the
humanized_vh/humanized_vlinto antibody developability — humanization can move charge and aggregation. - Re-number the humanized sequence with ANARCI.
- Confirm the mutations avoid the paratope so binding is preserved.
Read next
Was this page helpful?