Discover Targets From Omics

Slice a COVID-19 PBMC severe-vs-healthy cohort out of Arc Institute SE-167M-Human single-cell RNA atlas (167M human cells across tissues) (Hugging Face arcinstitute/SE-167M-Human; datasets.load_dataset('arcinstitute/SE-167M-Human', streaming=True) — slice by tissue/disease metadata before materialising.) (HF streaming loader, filter on disease + tissue=PBMC), analyse with Scanpy: cluster to identify cell types, run DE between COVID-severe vs healthy for each cluster, annotate top markers with MyGene.info, check OpenTargets for druggability, and query DGIdb for existing drugs targeting DE genes - which cell-type-specific markers are druggable, and do the hyperinflammatory monocyte markers suggest repurposing opportunities for approved JAK inhibitors or anti-IL-6 antibodies?